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Efficacy of Agmatine Nanoliposomes on Proliferation and Differentiation of Adult Mouse Neural Stem Cells
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M Jafari Barmak1   , H Bardania2 , A Ghanbari3 |
1- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2- Molecular Cell Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran , amirghanbari52@yahoo.com |
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Abstract: (1800 Views) |
Background & aim: Neurogenesis throughout the life of each individual must be continuous and dynamic so that the efficiency of different parts of the brain does not become weak and ineffective. On the other hand, protecting old neurons can also be a solution, as a result, synthetic materials that can simultaneously have both protective and neurogenic functions can be an apt option for targeted drug delivery in brain diseases and aging. In particular, agmatine, due to its role as a polyamine precursor and its ability to cross the blood-brain barrier while inhibiting nitric oxide synthase and having a beneficial protective and neurogenic role, has been reported to be a good option for targeted drug delivery in protection and neurogenesis. Therefore, the aim of the present study was to investigate the in vitro efficacy of agmatine nanoliposomes on the proliferation and differentiation of neural stem cells in the brain of adult male mice.
Methods: In the present experimental-in vitro study conducted in 2023, neural stem cells were first isolated from the subventricular zone of the adult mouse brain and proliferated and neurospheres were formed in the presence of epidermal growth factor and fibroblast growth factor. Cell viability was measured in six groups including the control group, a nanoliposome group, an agmatine group with concentrations of (25 and 50) µM, and nanoliposome groups treated with agmatine with concentrations of (25 and 50) µM by the MTT method. Also, the number of neurospheres, the number of cells resulting from each neurosphere, and the number of neuronal and glial cells following differentiation were counted. The collected data were analyzed using a one-way variance test.
Results: The cell survival of neural stem cells in the agmatine groups increased in a dose-dependent manner, with a significant increase in the 50 and 100 μM groups compared to the 10 μM agmatine group (**p<0.01). The mean number of neurospheres in the nanoliposome-agmatine 50 μM groups showed a significant increase compared to the liposome and control groups (*p<0.05, **p<0.01). The mean number of neuronal cells in the nanoliposome-agmatine 25 and 50 groups showed a significant increase compared to the liposome and control groups (***p<0.0001).
Conclusion: The present study indicated that agmatine and nano-liposomes containing agmatine increased the proliferation and differentiation of stem cells. This increase in the targeted method with liposomes was more effective at lower concentrations of agmatine, indicating the targeting and value of the liposomal system.
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| Keywords: Agmatine, Neural stem cells, Neurogenesis, Nanoliposome |
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Full-Text [PDF 777 kb]
(22 Downloads)
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Type of Study: Research |
Subject:
Anatomy
Received: 2024/09/8 | Accepted: 2024/12/16 | Published: 2025/05/6
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