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Background & Aim: Cancers are the second cause of death after cardiovascular diseases globally. Colorectal cancer is the third most prevalent cancer in the world. This cancer is the third leading cause of death in America and one of the most prevalent cancers of the gastrointestinal tract in Iran. The purpose of this study was to estimate the survival rate of patients with gastric cancer in Chaharmahal and Bakhtiari province, Iran.

Methods: The present observational (the analytical type) study was conducted using the longitudinal and survival study methods. The population study included all patients with gastric cancer in Chaharmahal and Bakhtiari province, Iran. The samples were selected by the census method and all the patients diagnosed with colorectal between the years 2000 to 2010 (estimated to be about 452 cases). After collecting the data and coding them, they were inserted into the SPSS 16 and STATA 12 software. To investigate the survival and the factors influencing the survival time of patients which was the main objective of this study, the Kaplan-Meier method was used. In order to compare the survival function in different subgroups, the log rank test or generalized Wilcoxon test was used.

Results: From the total 452 patients with colorectal cancer, 232 cases were male (51.3%) and 220 were females (48.7%), respectively. The mean age of patients was 59.12 ±19.99 years at the time of diagnosis. The median age of patients was 60 years. The probability of survival at 1, 5 and 10 years after diagnosis of colorectal cancer in both sexes using the Kaplan-Meier was 85.7%, 71.9,% 52.5% respectively.

Conclusion: Although the survival rate of patients with colorectal cancer in Chaharmahal and Bakhtiari province was at a desirable level, the need for further studies to identify prognostic factors is essential.

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Background & aim: Angiogenesis is associated with tumor growth and metastasis of tumor cells, this processes directly linked with the production of nitric oxide. In this study anticancer effects of hydroalcohoic extract of M. chamomilla and avastin (bevacizumab) were investigated via dimethyl thiazol diphenyltetrazolium bromide (MTT) cell viability assay and nitric oxide (NO) production level in colon cancer cell line (HT-29).

Methods: In the present experimental study, the HT-29 cell line was cultured in RPMI-1640 media supplemented with 10% (v/v) fetal bovine serum (FBS), 1% antibiotic solution (consisting of100 U/mL penicillin and 100 µg/ml streptomycin). After growing to a favorite confluent, 10⁴cells were seeded into separate 96-well culture microtiter plates and incubated at 37⁰C in an incubator with 5% CO₂ for 24 h prior to treatment. Every plate was treated with different   concentrations of the extract (1000, 1400, 1800, 2200, 2600 µg/ml of medium) and bevacizumab (100,200,300 µg/ml).  The production of NO was assessed by Griess reagent and the cell viability was determined by MTT assay. The results were compared by one-way ANOVA followed by Tukey-Kramer.

Result: The results of MTT assay indicated that the extract and bevacizumab anticancer effect is time and dose dependent. The highest percentage of cell death was observed after 48 h incubation which increased in the bevacizumab concentration (P<0.01). Fifty percent inhibitory concentration (IC50) of extract in 24 h and 48h was 1881 and 1669 µg/ml, respectively. Inhibition of nitric oxide (NO) production was maximum in 2600 µg/ml extract concentration.

                                                                                                                                             

Conclusion: The results of the present study demonstrated that bevacizumab and the hydroalcohoic extract of M. chamomilla had an inhibitory effect on NO production by HT-29. The production of NO by HT-29 was inhibited after treatment by the extract of M. chamomillamay contribute to angiogenesis via decreasing of NO production and has antimatastatic effect.

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Abstract
Background and Aim: Colorectal cancer (CRC) is the second most common cancer in developed countries.  More than 10% of CRCs are inherited and include HNPCC and FAP ligation syndrome. The MSH2 gene is located on chromosome 2 (p21) and consists of 16 exons. MSH2 is a protein that plays a role in restorative MMR after DNA replication. The MSH2 protein is attached to MSH6 or MSH3 and forms MutSα and MutSβ complexes that identify small and large deletion / insertion abnormalities, respectively. The main objective of the present research was to evaluate HRMA's ability and effectiveness in detecting tri-nucleotide deletion mutations and determine the frequency of these mutations in homozygous and heterozygous states in colon cancer samples compared to control group.
 
Methods: In the present study, a case control was carried out in 2016-2017 to detect the mutations of the three nucleotide deletions of GTG in the position of exon 12 of MSH2 gene. For this purpose, 50 samples of colorectal cancer with 50 healthy samples were studied. At first, genomic DNA was extracted from paraffin tissue samples and then examined by HRMA technique and direct sequencing of the triple nucleotide deletion mutant GTG. The controls used included a sequence of 96 bat games in wild type and 93 bp in the mutant mode. Equal portions of these two were used for the heterozygous state of this site. Finally, the number of samples in each group was compared in one way ANOVA and LSD mean comparison.
 
Results: The number of heterozygous samples for the site in colon cancer samples was significantly higher than that of homozygous consecutive homozygos. In general, the results of the present study indicated that the HRMA technique had the ability to separate and remove the pairs of homozygos and heterozygotes according to their melting temperature (Tm)
 
Conclusion: According to the results of this study, the frequency of removal of the three nucleotide GTGs in cancerous samples was significantly higher than healthy ones.
 
 

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Abstract                
Background & aim: one of the most common and disruptive symptoms experienced by cancer survivors is cancer related fatigue. This symptom can continue many years after cancer diagnosis, leading to reduced performance, quality of life, and significant socioeconomic outcomes. Therefore, the aim of this study was to evaluate the improvement of cancer-related fatigue in patients with breast and colorectal cancer who participated in the MBSR training program.
 
Methods: In the present clinical trial study, the statistical society included all patients with breast and colorectal cancer referred to Shahid Motahari Clinic and Amir Oncology Hospital (Shiraz, 2017-2018). 38 participants were selected through available sampling and randomly assigned to either experimental(n=19) or control(n=19) groups. The groups responded to the cancer related fatigue scale in pre-test and post-test. The results were analyzed using multivariate covariance (MANCOVA) analysis.
 
Results: the results of data analysis indicated that mindfulness-based stress reduction significantly decreased the mean scores of the fatigue subscales: Physical (F=141.49, p<0.0001), cognitive (F=66/23, p<0/0001), emotional (F= 58.44, P<0.0001), and overall fatigue score (F=5.66, P= 0.023) in the experimental group. That is, there was a significant difference between the two experimental and control groups in the mean of all dimensions of cancer related fatigue scale.
 
Conclusion: This study provided evidence that MBSR was effective in treating cancer-related fatigue in patients with colorectal and breast cancer. Therefor MBSR could be used as an effective intervention in medical centers and hospitals.