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Abstract                
 
Background & aim: Tissue engineering identifies degraded tissue components and provides rational solutions to improve and perform them. One of these approaches is to fabricate a mixed scaffold with polysaccharide and synthetic antioxidants for stem cells to be cultured inside. The aim of this study was to evaluate the potency of poly caprolactan-chitosan-tannic acid scaffold for proliferation of fibroblast cells.
 
Methods: In the present experimental study, polycaprolactane, chitosan powder and tannic acid scaffold were prepared for growth of fibroblast cells. Subsequently, the following groups were designed: Group 1: Polycaprolactane scaffold Group 2: Polycaprolactane-chitosan scaffold Group 3: Polycaprolactane-tannic acid scaffold Group 4: Polycaprolactane-chitosan-tannic acid scaffold. The human foreskin was prepared and the dermal layer fibroblast cells were isolated after laboratory tests, then the cells were placed in cell culture flasks with DMEM medium and stored in a 5% CO² incubator. Ten thousand cell fibroblasts were transferred to 96-well wells containing DMEM solution and scaffolds and then fibroblast cell proliferation and viability were determined by MTT assay and by SEM microscopy to determine the infiltration of fibroblast cells into scaffolds and also in order to review of the chemical groups in the polymers was performed using a FTIR spectrometer. The results were analyzed by the SPSS software; ANOVA and Tukey's post hoc test after the data were analyzed uniformly.
 
Results: The mean survival rate of fibroblast cells based on MTT assay at 24 h was significantly increased in the polycaprolactone-tannic acid scaffold group (p<0.05) compared to the polycaprolactone scaffold group (p<0.05). The results also indicated that the mean survival of cells based on MTT assay at 24 h was significantly increased in the polycaprolactone-chitosan-tannic acid scaffold group (p<0.05) compared to the polycaprolactone scaffold group (p<0.05). Moreover, the mean cell viability in the polyprolactone-chitosan scaffold was not statistically significant compared to the polyprolactone group.
 
Conclusion: Due to its hydrophilic properties and biocompatibility of chitosan and tannic acid, poly caprolactone-chitosan-tannic acid scaffold may be a suitable scaffold for the activity of fibroblast cells in the scaffold.  It can also be a good environment for the growth and proliferation of other cells.
 
 

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Background & aim: Considering the importance of the skin in regulating the temperature and maintaining body fluids and its prominent role in the defense system, it is important to improve any discontinuity in the skin, which is called a wound. Although limited and superficial wounds heal by themselves, the treatment of deep wounds requires drug therapy. Therefore, the aim of this study was to determine and prepare a topical cream containing the extract of the sumac plant (Rhus Coriaria L) and compare its effect with phenytoin in healing skin wounds in an animal model.

Methods:  In the present study conducted in 2021-2022, twenty-five New Zealand laboratory rabbits, with the weight range of 2500-3000 grams, were obtained from central animal house, Hamadan University of Medical Sciences. The rabbits were randomly divided into five groups: I) Control Group; II) Receiving Phenytoein cream (1%) as the medical therapy; III) Receiving the placebo cream base; IV) Receiving therapeutic cream containing standardized sumac extract (0.1% of tannic acid); and V) Receiving therapeutic cream containing standardized sumac extract (0.05% of tannic acid). Then, a wound, with dimension of 2*2 cm, was induced in the back of any rabbits and the appropriate topical therapy was administered twice daily on the rabbits. The effectiveness of therapy using creams containing sumac extract was determined using the extent of wound healing and the level of hydroxyproline. The obtained data were statistically analyzed using One-way Analysis of Variance (ANOVA).

Results: It was demonstrated that each one gram of standardized extract contained 2.74±0.86 milligram of tannic acid. The data achieved from the study, including the determination of hydroxyl-proline levels, percentage of wound healing, and the duration of remission, demonstrated significant efficiency in treatment with sumac-containing creams (either 0.1% or 0.05%). Histological studies revealed more condensed collagen fibrils and more reduction of inflammation cells in treatment groups receiving sumac-containing cream (either 0.01% or 0.05%) compared with the control group.

Conclusion: Sumac-containing creams, due to anti-oxidative properties and enhancing synthesis of hydroxyl-proline, can condense collagen fibrils which consequently cause improved wound healing.