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Introduction & Objective: Listeria monocytogenes is considered as a ubiquitous foodborne pathogen which can lead to serious infections, especially in newborns, elderly, pregnant, and immunocompromised people. The organism has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. Also L. monocytogenes forms biofilms on many food contact surface materials and medical devices. Development of biofilms on many surfaces is a potential source of contamination of foods that may lead to spoilage or transmission of foodborne pathogens. Materials & Methods: Biofilm formation of L. monocytogenes (RITCC 1293 serotype 4a) was investigated. Hydrophobicity of L. monocytogenes was measured by MATH method. Then biofilm formation of the organism was assessed at 2, 4, 8, 16 and 20 hours on stainless steel (type 304 no 2B), polyethylene and glass by drop plate method. Results: Results indicated that L. monocytogenes with 85% of hydrophobicity formed biofilm on each of three surfaces. Biofilm formation on stainless steel surfaces was significantly more than other surfaces (p<0.05). Conclusion: The ability of biofilm formation of L. monocytogenes on medical devices and food containers is very important as far as hygiene and disease outbreaks are concerned.

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Abstract

 

Background & aim: Entamoeba gingivalis and Trichomonas tenax are oral protozoa that could cause periodontitis and gingivitis. The present study was done to determine the prevalence of these two protozoa in people over 14 years with periodontitis and gingivitis.

 

Methods: In this descriptive, cross-sectional study, 540 patients referring Resalat Dental Clinic, Shahrekord were enrolled and assigned in two groups of 270 patients with periodontitis and gingivitis and270 healthy individuals. The prepared specimens were examined by extensive wet procedures, Gimsa staining and Trichorom staining. Data were analyzed by chi-square, Fisher’s exact test, and logistic regression in SPSSv.20.

 

Results: No E. gingivalis- and T. tenax-positive cases were seen in the healthy group. The prevalence of E. gingivalis and T. tenax was obtained 3% by extensive wet procedure, 1.9% by Trichoderma staining, and 0.7% by Giemsa staining respectively. By logistic regression model, none of variables of age, gender, place of residence, smoking, tooth brushing, flossing, and oral PH were associated protozoan infection of E. gingivalis and T. tenax (P>0.05).

Conclusion: In patients with periodontitis and gingivitis referred to the dental clinic, parasitic infections were attenuated to gingivialis and trichomoniasis vaginalis, and possibly other non-parasitic agents, including bacteria or other microorganisms, may play a role.

 

 

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Background & aim: The difference in the incidence of population transition in the provinces of Iran is accompanied by differences in the indicators of aging in them. The purpose of the present study was to determine the main causes of death of the elderly in the provinces of the country.
 
Methods: The present study was conducted in terms of quantitative method, in terms of applied purpose and in terms of implementation as a secondary analysis in 2021 as well as using death data by age cause during the 2011 to 2017. The cause of death data was taken from the current registration in the Civil Registration Organization and based on the international classification of the cause of death. This data was analyzed by gender and age groups in the elderly population. The Lifespan Age Index (YLL), as a percentage per thousand population, was used to measure the mortality of each disease. The rate of death and the number of years of life lost allow comparison over time, sex and age at the provincial level as standard. The collected data were analyzed using Excel software and drawing appropriate tables and graphs and indicators such as sex ratio, relative difference and age-specific rate.
 
Results: The ratio of elderly deaths to the total number of deaths in the provinces of the country varies from 40 to 72% and displays the difference in the situation of the provinces in the epidemiological transition. The share of causes of death correspondingly varies in the provinces of the country and during the two sexes. In less developed provinces, premature death is more common for men. Deaths from infectious and parasitic diseases are still present in less developed provinces, and the predominant cause of death in the provinces, especially in developed areas, is non-infectious and parasitic diseases.
 
Conclusion: The provinces of the country have different climatic, developmental and demographic conditions that have led to different occurrence of health conditions in them. It is necessary to enter into the aging phase of the population and the prevalence of chronic diseases among the elderly, appropriate health policies at the provincial and regional levels in the country to be developed.

 

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Background & aim: Population has always been considered as one of the most fundamental issues in policy-making and futuristic planning. Population futures research is also a process for making better decisions and planning by looking at distant horizons, futures that are broadly related to values, beliefs, and patterns of behavior. One of the major demographic issues, mainly in the world, is the issue of population aging and its consequences. As a result, the purpose of the present study was to determine and study the future study of the elderly population of Kohgiluyeh and Boyer-Ahmad province up to 2050 and their health costs.
 
Methods: In the present descriptive study conducted in 2019, a documentary study of the general census data of Iran Statistics Center in 2016 based on possible scenarios of fertility, migration and death of Mir using a combined method of forecasting was used. Population was analyzed in Spectrum and Mortpak demographic software and the aging status of the province's population up to 2050 horizon was analyzed.
 
Results: Based on the probabilistic scenario calculations (total fertility 1/2), important population indicators such as number of elderly, aging index and dependence ratio of elderly population in Kohgiluyeh and Boyer-Ahmad province for 2050 were estimated at 259017 people, 81.92 and 25.8, respectively. Another finding of the research indicated that from 2016 to 2050, the index of dependency ratio of the elderly will increase relatively much. Accordingly, from 6.8% in 2016 to 26.6% (low growth), 25.8% (medium growth), 25.5% (high growth). The sex ratio of the elderly in the province will reach 95 in 2050, which indicated that the elderly population of the province is moving towards femininity. Life expectancy for the elderly at the age of 60 is also projected at 2330, equal to 23 years. This index has increased significantly compared to 2016. Another finding of the present study revealed that the average direct cost of health is higher for men until about 2025, and after that, due to the increase in older women, the cost of women's health is higher.
 
Conclusion: Considering the rapid pace of declining fertility in Iran over the last two decades, it is predicted that the movement towards population aging will accelerate and based on the observed trends in fertility and mortality and a look at the most likely ways to change them in the future, the age structure was estimated. The population of Kohgiluyeh and Boyer-Ahmad is getting older, in other words, the share of the population in the age group under 30 decreases and the share of the population over 30 increases, considering the age structure of the population It is necessary.
 
 

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Background & aim: Diabetes is associated with cardiovascular and metabolic disorders. Although the favorable role of physical activity and antioxidants in people's health has been reported, the simultaneous effect of HIIT and crocetin on angiogenesis in heart tissue is not well known. The aim of the present study was to investigate the effect of eight weeks of intermittent aerobic training with the consumption of crocetin extract on some indicators of angiogenesis in the heart tissue of prediabetic and diabetic aged rats.

Methods: the present experimental study was conducted in 2022. Forty-five elderly male mice with diabetes aged 14-16 weeks and weighing 30-35 grams were divided into groups; Pre-diabetic, pre-diabetic+aerobic exercise, pre-diabetic+crocetin, pre-diabetic + aerobic + crocetin, diabetic, diabetic + aerobic exercise, diabetic + crocetin and diabetic + aerobic + crocetin. In order to investigate the effect of modeling on the variables, 5 rats were included in the healthy control group. HIIT aerobic exercise was performed for eight weeks, five sessions per training week, and a dose of 40 mg/kg streptozotocin (STZ) was injected. And the mice received crocetin at the rate of 30 mg/kg per day by intraperitoneal injection. The expression level of FGF and No genes was measured by Real time RT-PCR technique. The collected data were analyzed using one-way analysis of variance and Tukey's post hoc test.

Results: The expression values of FGF-2 in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement were significantly higher than other groups (P≥0.05); NO gene expression values in the pre-diabetes group + aerobic exercise + crocetin supplement and diabetes group + aerobic exercise + crocetin supplement were significantly lower than other groups (P≥0.05). The insulin and glucose levels in the pre-diabetes group + aerobic exercise + crocetin supplement and the diabetes group + aerobic exercise + crocetin supplement were significantly lower than other groups (P≥0.05).

Conclusion: It seemed that interval aerobics and crocetin were effective both alone and synergistically in improving angiogenesis in heart tissue with pre-diabetes and diabetes. Therefore, the use of periodic aerobics and crocetin is recommended in pre-diabetes and senile diabetes.


 

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Background: Cellular senescence is an irreversible process in cells that is affected by various factors such as oxidative stress and inflammation, and the importance of this issue increases in its effect on neural stem cells. Fluvoxamine at appropriate concentrations provides proliferation and differentiation of neural stem cells into glial and neurons and modulates inflammatory factors. Therefore, considering the mechanism of the aging process on neural stem cells, this study investigates the effects of fluvoxamine in culture.
Materials and methods: In this experimental study, neural stem cells were first isolated from the subventricular zone of the adult male mouse brain. Neutrosphere cell colonies were formed in the presence of epidermal growth factor and fibroblast growth factor. The survival of neurospheres in fifteen groups including a control group, treatment group with 20 μM D-galactose concentration, and fluvoxamine treatment groups with different concentrations and combined D-galactose with fluvoxamine groups was measured by the MTT method. Also, the number of neurospheres, the number of cells resulting from each neutrosphere cell, and the number of senescent cells were counted. The collected data were analyzed using GraphPad software and a one-way ANOVA test.
Results:  The average data from the survival of neural stem cells in the 25 and 50 nM fluvoxamine groups significantly increased compared to the control group and decreased significantly with increasing doses (p<0.001). Also, the survival of cells in the D-galactose group significantly decreased compared to the control group (p<0.01), which showed a significant increase in the treatment with low-dose fluvoxamine compared to the D-galactose group (p<0.001) and a significant decrease in the high-dose groups. The number of neurospheres in the D-galactose group was significantly decreased compared to the control group (p<0.001). Also, the number of neurospheres in the D-galactose group with 50 nM fluvoxamine compared to the D-galactose group was significantly increased (p<0.01). The number of BrdU-positive cells in the fluvoxamine 100 group showed a statistically significant increase compared to the control group (p<0.001). The number of BrdU-positive cells in the di-galactose group also showed a statistically significant decrease compared to the control group (p<0.01). The combination treatment of di-galactose with fluvoxamine at low doses increased the number of BrdU-positive cells compared to the di-galactose group. The average number of neural stem cells in the di-galactose groups decreased significantly compared to the control group, and the combination of di-galactose with fluvoxamine at doses of 50 showed a statistical increase compared to the di-galactose group (p<0.001). The average number of senescent cells increased significantly with increasing fluvoxamine doses compared to the control group. Also, treatment with di-galactose increased the number of senescent cells compared to the control group, and treatment with di-galactose and fluvoxamine at concentrations of 25 and 50 nM showed a statistically significant decrease in the number of senescent cells compared to the di-galactose group (p<0.0001).
Conclusion: The results of this study indicate that fluvoxamine, prescribed for the treatment of major depression and obsessive-compulsive disorders, while increasing the survival and viability of cells at low doses, exhibits significant toxicity at higher doses in a dose-dependent manner. Furthermore, it elevates aging-related factors and induces aging in neural stem cells under ex vivo conditions. In other words, it reduces the survival of neural stem cells, the number of neurospheres, and the number of cells derived from each neurosphere, while simultaneously increasing the number of aged cells. These findings necessitate further investigations in both in vivo and in vitro environments.