---

Abstract Background & aim:Foodborne disease, a major health and economic problem in industrialized and non-industrialized countries.The purpose of this study was to investigate Salmonella contamination of eggs by native province kohgiloyeh va Boyerahmad by PCR and evaluation of their drug resistance. Methods: This cross-sectional study-descriptive study of 210 eggs collected from native Kohgiluyeh va Boyerahmad done. Biochemical tests for identification of bacteria was isolated. Salmonella bacteria have suspected reactions were tested by PCR with specific primers invA genes were examined. Results: The results showed that 14 number of eggs (6/66 percent) were contaminated with Salmonella genus. Dehdasht area of highest contamination and less pollution Charusa areas, Dyshmuk, Lndeh and was Basht And no significant correlation was found between the type and extent of contamination and the region.The antibiotic resistance of most resistance to penicillin (100%) was observed.This study uses data from the nineteenth and application soft ware spss version microsofte office 2007-square test and Fisher were analyzed. Significant level of p>0/05 was considered. Conclusion: Microbial agents such as Salmonella can cause food spoilage and disease are. Resistance in Salmonellais recommended to avoid the in discriminate use of antibiotics in live stock and poultry should be avoided. Key words: Salmonella,Egg,drug resistance, invA, PCR.

---

Introduction & aim: To achieve high performance and health, it’s better to use additives in the human diet which have beneficial effects on good bacteria and damaging effect on the harmful bacteria. For this purpose, effects of Chavill extract on growth, viability and death of lactobacillus and salmonella, in skimmed milk and imaging gastric-intestine media were studied in vitro conditions. Methods: This study was investigated in two completely randomized experiments with three levels of Chavill extract. In the first experiment, ability of the Chavill extract in Skim Milk medium was examined to survey survival, proliferation and death of beneficial and pathogenic gut bacteria. The second experiment which was down in the simulation of simulated gastric juice and simulated small intestine juice, the effect of Chavill extract on survival, proliferation and death of the bacteria were investigated. Treatments in both of experiments were three levels of Chavill extract (0, 1, and 3 %) for three probiotic bacteria species. Data were analyzed with SAS 9.1 software and their means were compared by Duncan’s Multiple Range test at a significance level of 5 %. Results: By increasing of Chavill extract concentration to 1%, probiotic bacterial counts significantly increase compared to control treatment and the differences were significant and the count of Salmonella typhimurium difference with control significantly decreased. Using 3% Chavill extract compared to 1% extract, increased number of Lactobacillus acidophilus and Lactobacillus plantarum, decreased number of Lactobacillus casei, inhibit growth of Salmonella typhimurium bacterium and block growth of this bacterium. The second experiment on simulated gastric juice showed that numbers of Lactobacillus acidophilus and Lactobacillus plantarum bacteria increased and Lactobacillus casei and Salmonella typhimurium decreased. Also, findings of bacterial survival on simulated small intestine juice showed that there was no significant difference in bacterial on Lactobacillus acidophilus and Lactobacillus plantarum. Conclusion: Results showed that Chevill have good effects on binifical bacteria of digestive system and increases the number of beneficial bacteria and have harmful effects on pathogenic bacteria. It seemed that this plant could be used in diets and also for diseases causing imbalances of normal flora in the gastrointestinal tract. .