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Showing 2 results for Hamoonnavard
M Rabia Moghadam, H Rezvan, A Nourian , S Hamoonnavard,
Volume 29, Issue 5 (10-2024)
Abstract
Background & aim: Cutaneous leishmaniasis is a tropical and treatment-resistant disease which the range of treatment strategies based on effective plant substances has been expanded due to the side effects of standard druge. Therefore, the aim of the present study was to determine and investigate the histopathological changes caused by Leishmania major in BALB/c mice treated with aqueous extract of garlic.
Methods In this experimental study, which was conducted in 2018 at the Faculty of Veterinary Medicine of Bou Ali Sina University,, 36 female BALB/c mice with an average weight of 30g were equally divided into 6 groups and the standard strain of Leishmania major parasite was injected into 5 groups. Three groups were treated with aqueous garlic extract orally and topically, and three groups were treated as control, without treatment and received Glucantim as drug. The size of the wound in five groups were evaluated weekly, and after the 90-day of treatment, skin tissue around the wound, lymph nodes, spleen, and liver were taken for histopathologic evaluation. The collected data were analyzed using statistical tests of two-way analysis of variance (pvalue ≤ 0.05).
Results: A significant reduction in wound size was observed in the groups receiving the garlic extract orally (p≤0.0001). The structures of the lymphoid organs differed in these two groups in inflammation, granuloma, and lymphocyte populations compared to the control groups and even to the group receiving garlic topical ointment, and the healing process was observed in them, also, wound healing was significantly accelerated in the group receiving oral garlic extract before parasite injection (p≤0.05). Histopathological changes in the group receiving garlic ointment also showed evidence of wound healing, but at the end of the treatment period, it was less effective than the group receiving oral garlic (p≤0.05).
Conclusion: The oral extract of garlic changes the inflammatory reactions in the wound site of cutaneous leishmaniasis towards repair and accelerates the healing process of the wound. Garlic extract compounds can be considered as a natural treatment option in treatment-resistant Leishmania infection.
Sahar Hamoonnavard, Hossein Rezvan, Maral Maleki, Hamidreza Heydarianpour, Behnaz Bazargani-Gilani, Fatemeh Behdarvand,
Volume 30, Issue 1 (1-2025)
Abstract
Introduction: The side effects and resistance to chemical treatment of cutaneous leishmaniasis have led to intensive research into the use of herbal compounds for the treatment of this disease. The antimicrobial properties of Carthamus tinctorius and Matricaria chamomilla extract have been investigated on many microorganisms. The aim of this study was to investigate the anti-leishmanial effect of these two plants on the promastigotes of the Leishmania major.
Materials and methods: The standard strain of Leishmania major was obtained from Pasteur Institute of Iran. Ethanol extract of safflower and water-ethanol extract of chamomile were prepared at concentrations of 15, 25, 50, 75, 100 and 200 μg/ml and exposed to promastigotes cultured in 96 plates in triplicate and incubated for 24 hours. Using colorimetric method and tetrazolium reduction, the viability of promastigotes was evaluated by absorbance at 490nm. The inhibitory concentrations(IC) IC10, IC50, IC80 each of the extracts were calculated with Graphpad Prism 8 statistical software.
Findings: Exposure to safflower and chamomile extracts at all concentrations decreased parasite survival(p≤0.05), such that inhibitory concentrations was IC10: 21.10ug/ml, IC50: 3.85ug/ml and IC80 1.31ug/ml for safflower and inhibitory concentrations was IC10: 24.27ug/ml, IC50: 17.69ug/ml, IC80: 14.48ug/ml for chamomile extract. Concentrations of more than 15 μg/ml and more than 25μg/ml had significant anti-leishmanial effects compared to the untreated control (p≤0.05).
Conclusion: The anti-leishmanial effect of safflower extract and chamomile extract on Leishmania major was remarkable at low concentrations and can be investigated as a herbal compound in the in-vivo model of cutaneous leishmaniasis.
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