|
|
|
 |
Search published articles |
 |
|
Showing 24 results for Ghasemi
Ss Saei Dehkordi, H Tajik, M Moradi , A Jafari Dehkordi, S Ghasemi,
Volume 14, Issue 3 (10-2009)
Abstract
Introduction & Objective: Rosmarinus officinalis L. as a member of the Lamiaceae family and lysozyme as a natural antibacterial agent is important in food microbiology, because of its characteristics. The aim of the present study was to determine the chemical composition and anti-listerial activity of Rosmarinus officinalis essential oil (REO) alone and in combination with lysozyme for enhancement of anti-listerial activity of both substances.
Materials & Methods: Rosmarinus officinalis L. was purchased from a local grocery store at Shahrekord and was identified by the Institute of Medicinal Plants, ACECR. The air-dried aerial parts were subjected to hydrodistillation using a Clevenger apparatus to obtain essential oil and yielded oil was analyzed by GC/MS. Antibacterial activity (on basis of Minimum Inhibitory Concentration (MIC) of REO was studied separately and in combination with unheated lysozyme (L) and heat-treated lysozyme (HTL) on Listeria monocytogenes at different pH (5, 6 and 7) by a micro-broth dilution assay. The collected data were analyzed by SPSS software.
Results: In the current study, 98.05% of constituents of the essential oil were identified. The major components were α-pinene (14.06%), 1,8-cineole (13.62%), verbenone (11.2%), camphor (10.51%), borneol (7.3%), 3-octanone (7.02%), camphene (5.46%) and linalool (5.07%). The inhibitory action of REO was stronger at lower pH especially 5 (MIC=225 μg/mL). Inhibition by L at pH 5 was 640 μg/mL but no inhibition was seen at pH 7. HTL resulted in more effective inhibition than L, especially at pH 5 and heat-treatment 80˚C (MIC: 160 μg/mL).
Conclusion: Combination of L + REO and particularly HTL + REO was led to enhancement of bacterial inhibition. It was concluded that REO by the identified chemical composition was effective alone or in combination with L or HTL on Listeria monocytogenes as a food-borne pathogen.
M Taherzadeh Ghahfarrokhi , E Farrokhi, A Shirmardi , S Ghasemi, M Abolhasani , F Azadegan , S Reisi, M Reisi, G Banitalebi , M Hashemzadeh Chaleshtori ,
Volume 14, Issue 4 (1-2010)
Abstract
Introduction & Objective: Hearing loss is a common disease affecting millions of people worldwide. Hearing loss can be caused due to genetic or environmental factors or even both. The genetic of hearing defect is highly heterogeneous and more than 100 genes are predicted to cause this disorder in humans. A newly identified gene (DFNB59) has been shown to cause deafness in some populations. Here we report mutation analysis for DFNB59 gene in 88 genetic non-syndromic hearing loss subjects.
Materials & Methods: In this descriptive-lab based study which was conducted at the Cellular and Molecular Research Center of Shahrekord University of Medical Sciences, DNA was extracted from the peripheral blood samples using standard phenol chloroform procedure. Mutation analysis for DFNB59 gene was performed using PCR-SSCP/HA protocol. The suspected DFNB59 which was detected as shifted bands on PAGE were then confirmed by direct sequencing strategy.
Results: Two DFNB59 polymorphisms including c.793C>G and c.793C>T were detected in 8 and 1 deaf subjects respectively.
Conclusion: We conclude that there is no association between DFNB59 mutations and deafness in the studied patients in the region.
H Shirzad , A Sedaghat , S Ghasemi , M Shirzad ,
Volume 15, Issue 1 (4-2010)
Abstract
Introduction & Objective: Wound healing is the process of repairing following injury to the skin and other soft tissues. Following injury, inflammatory response occurs and the cells below the dermis begin to increase collagen production, later on, the epithelial tissue is regenerated. Royal jelly (RJ) is a bee product. There are many reports on pharmacological activity of RJ on experimented animals. The purpose of this study was to investigate the effect of RJ on the induction of wound healing of sterile incision in Balb/C mice.
Materials & Methods: In this experimental study which was conducted at Shahr-e-kord University of Medical Sciences in 60 female Balb/C mice (8 weeks old) were selected. The mice were anesthetized with ether. The dorsal fur of the animals was shaved and sterilized with alcohol, and then a longitudinal para vertebral full thickness incision of 10mm long was made. The animals were then divided into six equal groups. In group one (negative control), nothing was applied to the wound. Group 2 (positive control) was treated with nitrofurazon ointment, group 3 was treated with RJ 200 mg/kg daily, group 4 was treated with RJ 200 mg/kg every two days, group 5 was treated with RJ 300 mg/kg daily, group 6 treated with RJ 300 mg/kg every two days. Royal jelly was topically used on the wounds. The wound length was measured with vernier capilar every two days until the complete healing was occurred. The data were analyzed with SPSS version 11.5 using Kruscal Walis tests.
Results: There was a significant difference between groups 1, 2 with the other groups (p< 0.015). RJ promoted wound healing activity significantly in group 3, 5 compared to negative and positive control groups. There was no significant difference between the dosage of 200mg/kg with 300 mg/kg of RJ (p>0.015).
Conclusion: The results of this study indicated that daily application of RJ possesses betters wound healing effects than nitrofurazon.
M Emamghoreishi, F Ghasemi ,
Volume 16, Issue 6 (2-2012)
Abstract
Background & aim: In Iranian traditional medicine, Crocus sativus L. has been defined as an exultant plant. The present study was undertaken to determine the effect of subchronic administration of aqueous and hydro-alcoholic extracts of Crocus sativus on mice.
Methods: The effect of subchronic i.p. administration of different doses of the aqueous extract (50, 100, 200, 400 mg/kg or water) and the hydro-alcoholic extract (100, 200, 400, 800 mg/kg or water) of Crocus sativus stigma on immobility, climbing, and swimming behaviors were evaluated in the forced swimming test in mice. Fluoxetine (20 mg/kg) and imipramine (15 mg/kg) were used as reference drugs. Additionally, the effect of both plant preparations on spontaneous activity was examined. The collected data was analyzed using One-way ANOVA.
Results: The aqueous extract at doses of 50 and 100 mg/kg produced a significant reduction in immobility along with an increase in climbing behavior which is similar to those which have been observed with imipramine. The hydro-alcoholic extract did not show significant effects on immobility, climbing and swimming behaviors of all studied doses, compared to control group. The aqueous extract of all studied doses and the hydro-alcoholic extract at dose of 1600 mg/kg decreased spontaneous activity.
Conclusion: The results of this study suggests that the aqueous, but not hydro-alcoholic, extract of Crocus sativus stigma from Estahbanat in Fars province, in subchronic administration possess an antidepressant-like activity which may be mediated through norepinephrine system.
S Mohebbi , A Mohammadi , N and Ghasemi ,
Volume 16, Issue 6 (2-2012)
Abstract
Background & Aim: The aim of this study was to compare the maladjustment indicators using Machover Draw-A-Person Test (DAP) and behavioral disorders between orphan and normal children aged 6-11 in Tehran and Shemiranat district, Tehran province, Iran 2010.
Methods: The present study was a case-control study. The sample included 84 children aged 6-11 years old. Forty two children were orphan (22 boys and 20 girls) and other 42 were non orphan (22 boys and 20 girls). They were enrolled by use of accessible sampling and were assessed by Machover Draw-A-Person Test and Ratter’s behavior disorder scale (teachers form). Their behavior was scored by their teachers. The data was analyzed by the use of descriptive statistics and inferential statistics (independent t, analysis of variance)
Results: Results of this study showed that maladjustment indicator of DAP in orphan children (Mean=6.43 ± 3.35) is more than non orphan children (4.50 ± 2.48) and the difference was statistically significant (p<0.004). Moreover, behavioral disorders in orphan children (Mean=15.81 ± 11.16) is more than non orphans (Mean=6.40 ± 6.14) this difference was also statistically significant. (p<0.0001)
Conclusion: The present study revealed that the maladjustment indicator of DAP and behavioral disorders in orphans is more than non orphan children.
A Amiri , A Ghasempour , S Fahimi , A Abolghasemi , E Akbari, A Agh , A Fakhari ,
Volume 17, Issue 1 (4-2012)
Abstract
Background & Aim: Practical emotional factors involve in disorders such as obsessive-compulsive disorders. The aim of the present study was to compare the expression of emotion facial recognition in patients with obsessive-compulsive disorder (OCD) and average people.
Methods: The present study was a cross-sectional and ex-post facto investigation (causal-comparative method). Participants were twenty clients suffering from obsessive-compulsive disorder (clients referred to Tabriz Bozorgmehr Special Center) and 20 normal people which were selected through the available sample method. Data were collected through a structured clinical interview and the Recognition Emotion Facial States Test. Multivariate analysis of variance, Chi-square and independent t-test were conducted to analyze the data.
Results: The findings showed that there was significant difference between the two groups in terms of recognition of facial expression of emotion (P<0.05). Accordingly, normal controls, in terms of these variables, were better than people with OCD.
Conclusion: This Study indicated that defect of recognition of emotion facial expression is constructible which can have a significant role in intensifying obsessive-compulsive disorder precipitate symptoms.
M Nikseresht , T Rasti , M Jafari Barmak , H Ghasemi Hamidabadi , Z Rezaei, F Dehghani , R Mahmoudi ,
Volume 18, Issue 8 (5-2013)
Abstract
Abstract
Background & aim: Vitrification is a simple and ultra rapid technique for the conservation of fertility. Improving pregnancy rate associate with the use of cryopreserved oocytes would be an important advanced in human assisted reproductive technology (ART). The purpose of this study was to evaluate survival, oocytes maturation and embryo development to the blastocyst stage after vitrification of oocytes germinal vesicle-stage and multi stage
Methods: In the present experimental study, germinal vesicle oocytes with or without cumulus cells were transferred to vitrification solution containing 30% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose, either by single step or in a step-wise way. After vitrification and storage in liquid nitrogen, the oocytes were thawed and washed twice in culture medium TCM119, and then subjected to in vitro maturation, fertilization, and culture. Data analysis was performed by using One-way variance and Tukey tests.
Results: Oocytes survival, metaphase 2 stage oocyte maturation, fertilization and embryo formed blastocyst in vitrification methods multistage were significantly higher than the single step procedure (P<0/05)
Conclusion: The Germinal vesicle stage oocytes vitrified with cumulus cells and stepwise procedure had positive effect on the survival, maturation and developmental rate on blastocyst compared to oocytes without cumulus cell and single step procedure.
Key words: Germinal Vesicle Oocyte, Blastocyst, Vitrification, Ethylene glycol
T Archin , E Afzalian, M Kargar, Y Ghasemi,
Volume 18, Issue 10 (1-2014)
Abstract
Abstract
Background and aim: β-lactamase enzymes producing bacteria ESBL have spread widely throughout the world. The production of enzymes induces bacterial resistance to a wide range of antibiotics which is leading to the limitation of infection control and correct treatment. The aim of the present study was to investigate patterns of antibiotic susceptibility to antibiotics and the presence of β-lactamase genes SHV, TEM, CTX-M, in Klebsiella pneumoniae isolates from clinical specimens of intensive care.
Methods: Susceptibility of isolated bacteria against 10 antibiotics was determined by agar disk diffusion method according to the CLSI guidelines. The strains (DDST) were examined for the presence of the spectrum β-lactamase enzymes. Using E-test, the minimum inhibitory concentration (MIC) of the antibiotic was determined to cefotaxime. Moreover, SHV, TEM, CTX-M genes were identified by, Multiplex PCR method, and some of them were sequenced.
Results: The antibiotic resistance against 10 antibiotics was determined. The highest percentage of isolates was resistant to ampicillin (100%) and sensitivity to imipenem was 1.66%). In this study, the majority of strains produced ESBL (60%). TEM gene in 34.38% and all three genes (TEM and SHV and CTX) at 33.13% of isolates were observed.
Conclusion: The present study showed that the K. pneumoniae producing ESBL in patients in ICU are common. Therefore, the use of procedures and policies for infection control in hospitals and especially ICU is necessary.
Key words: Klebsiella pneumoniae, ESBL, Multiplex PCR, antibiotic sensitivity
R Ahmadi, N Amini, A Ghasemi, A Seif,
Volume 19, Issue 8 (11-2014)
Abstract
Background & aim: Studies show that amphetamines can influence the hypothalamic-pituitary-thyroid axis. The aim of this study was to investigate the effects of Shisheh (met-amphetamine) on serum levels of T3, T4 and TSH in male rats.
Methods: In this laboratory experimental study 25 male Wistar rats were randomly divided to control, saline receiving, 2mg/kg, 4mg/kg, and 6mg/kg of Shisheh receiving groups of 5 rats in each. The injection was carried out intraperitoneally once a week for 6 weeks. After serum collection, T3, T4 and TSH levels were measured using ELFA method and the data were statically analyzed using ANOVA.
Results: Serum level of T3 and T4 was not significantly changed in rats receiving 2mg/kg of Shisheh compared with control animals. Serum level of T3 and T4 was signifintly increased in animals receiving 4mg/kg and 6mg/kg dose of Shisheh compared with the control rats (P<0.05 , P<0.001, respectively). Serum TSH level was significantly increased in rats receiving 2mg/kg, 4mg/kg, and 6mg/kg of Shisheh compared with control animals (P<0.001).
Conclusion: Our results indicate that Shisheh injection can enhance thyroid gland activity via acting on pituitary-thyroid axis, resulting in increased serum T3, T4 and TSH.
F Alizadeh, M Azarnia, G Mirabolghasemi , P Karampoor,
Volume 20, Issue 1 (4-2015)
Abstract
Abstract
Background & aim: Polycystic Ovary Syndrome (PCOS) is one of the most common endocrine disorders which affect 5-7 % of women in their reproductive age. Heracleum persicum (Golpar) contains compounds with anti-inflammatory, antioxidant, and antimitosis properties. The purpose of this study was to evaluate the therapeutic effect of ethanol extracts of Heracleum persicum fruit on polycystic ovary syndrome in rats.
Methods: In the present experimental study, thirty rats (200±20gr), were injected with 2 ml of estradiol valerate subcutaneously and six other rats were considered as the control group (intact). Sixty days after injection, the rats were divided into control, sham and experimental groups. The experimental group was treated with 200, 400 and 800 mg/kg BW of of Golpar extract interaperitoneally for ten days. The serum levels of LH, FSH, estradiol and testosterone were measured by ELISA and each serum were analyzed using ANOVA.
Results: A significant decrease was seen in serum levels of LH (P< 0.05), estradiol (p< 0.01) and testosterone (p< 0.01) in three experimental groups compared with the control group. Whereas, a significant increase in the mean FSH (p< 0.05) concentrations was observed in the groups receiving high dose.
Conclusions: Due to the decreasing effect of Heracleum persicum (Golpar) extract on LH, estradiol and testosterone levels and increasing in FSH level in PCOS rat, it seemed that the Golpar had a therapeutic effect on patients with this disease.
S Jalili Nikoo , Sh Kharamin , R Ghasemi Jobaneh, Z Mohammad Alippoor ,
Volume 20, Issue 4 (7-2015)
Abstract
Background & aim: family and individual factors are involved in addiction potential. The aim of this research was to investigate the role of family resiliency (family communication and problem solving, utilizing social and economic resources, maintaining a positive outlook, family connectedness, family spirituality, ability to make meaning of adversity) and mindfulness in addiction potential of students. Method: the research method used in the present study was correlation. A number of 399 students from Yasuj University in 2012-13 academic years were selected through a multi- stage cluster sampling method and responded to the Iranian scale of addiction potential, family resiliency questionnaire, and short form of Freiburg mindfulness inventory. Collected data were analyzed using Pearson correlation coefficient and Simultaneous regression. Results: A negative and significant correlation was seen between family resiliency and its dimensions with addiction potential (P>0.01). Between mindfulness and addiction preparation, a significant and negative correlation was observed (P>0.01). The results of simultaneous regression analysis showed that family residency and mindfulness could significantly predict 13% of variance of addiction potential. Conclusion: The results of this study indicated the importance of family resilience and mindfulness as personal and family variables in preparing addiction. Therefore, the family resilience and mindfulness skills training could decrease the addiction potential among students.
Z Rezaei, S Jalili Nikoo, N Nejati Nezhad, R Ghasemi Jobaneh, Kh Nooryan,
Volume 20, Issue 5 (8-2015)
Abstract
Background and Aim: Many problems are associated with infertility diagnosis, especially for women. Resiliency is one of the strategies which may reduce psychological distress of infertile women. The aim of current research was to study the effectiveness of existential psychotherapy in increasing the resiliency of infertile women. Method: The design of the present study was a semi-experimental research with pretest and posttest with control group. Statistical population consisted of all infertile women of Dehdasht, Iran, in the summer of 2014. Samples were selected at first by available sampling method and after completing resiliency questionnaire, and obtaining score for enter to research, were placement using random sampling method in two experimental and control groups (N = 8 per group). The experimental group participated in 8 sessions of group counseling based on existential approach and control group received no intervention. The gathered data were analyzed using covariance analysis. Results: The results showed that significant differences between the pre-test and post-test scores of the experimental group existed. This difference was significant at the level of 0.01. Therefore, it seemed that existential psychotherapy increased the resiliency of infertile women. Conclusion: The results revealed that existential psychotherapy increased resiliency of infertile women and interventions based on this approach will lead to the improvement of the mental health.
A Rahgozar, H Ghasemi Hamidabadi, S Shokri, A Moayeri, A Esmaeilnejhad Moghaddam,
Volume 20, Issue 9 (12-2015)
Abstract
Background & aim: Nandrolone decanoate is one of the most drug Anabolic-androgenic steroids (AAS). On the other hand, human chorionic gonadotropin (hCG) induce secretion of testosterone and increased androgen production. The main aim of this study investigated the effects of mentioned hormone on destructive effects of drug AAS in cells and tissues of male reproductive system.
Methods: In the present laboratory-experimental study, male Wistar rats were divided into 5 groups of three. Treated groups received 10 mg/kg/weekly of Nandrolone (Nd) for eight weeks, hormone group (H) rats received 500 IU weekly (IM or intra muscular) of hCG for 8 weeks, Nandrolone plus hCG group (Nd – H) received Nandrolone solvent or peanut oil as a vehicle or sham (Sh) and Control (CO) without any injection. Sperm parameters such as motility, count and morphology were evaluated after 8 weeks by light microscopic. In addition, percentage of sperm viability was prepared using Eosin-nigrosin. Moreover, testes, Epydidim tail, prostate and seminal vesicle were weighted by laboratory scales. Statistical analysis was carried out by one-way ANOVA.
Results: The percentage of progressive motile sperm was decreased in Nd and Nd - H groups in comparison to the control and sham groups. The percentage of normal sperm morphology was not significantly decreased in treated groups compared to the control and sham groups. In addition, the results of sperm morphology indicated that a high percentage of abnormal sperm morphology (Tailless and Coil) was seen in the Nd experimental group. Additionally, the viability percentage was significantly decreased in the Nd group in comparison to the other groups. The testes weight was significantly decreased in the Nd group compared to the control and sham groups.
Conclusion: The hCG had positive effects on the destructive effects of Nandrolone on sperm parameters of adult rats and decreased negative effects of Nandrolone.
S Jalili Nikoo , Z Karimi , N Nejatinejad , B Bahmani , M Naghiaee , R Ghasemi Jobaneh ,
Volume 21, Issue 10 (1-2017)
Abstract
Abstract
Background and aim: With an aging population, considering the factors affecting the quality of life more than ever is necessary. The aim of current research was to investigate the effectiveness of cognitive existential therapy on quality of life of elderly people.
Methods: The current research is semi experimental with pre and post test with control group. Statistical population of research consists of all elderly people in Kahrizak nursing homes. In the first phase, the participants were selected through purposive sampling method and after responding to the quality of life questionnaire and obtaining score for enter to research they were divided in two groups of experimental and control (N = 12 per group) using random sampling method. The experimental group participated in 10 sessions of group counseling based on cognitive- existential approach and control group received no intervention. The gathered data were analyzed using covariance analysis.
Results: There was no difference between pre-test and control groups, but the mean scores of post-test experimental and control groups were statistically significant. and cognitive group therapy improves quality of life is (p=0.001). Therefore it seems that cognitive-existential group therapy increase quality of life of elderly people.
Conclusion: Cognitive Existential Group therapy utilizes concepts such as death, meaning, cognitive distortions and responsibility could increase the level ofquality of life of elderly people. Thus interventions based on this approach could be useful in improving the quality of life.
Sh Ghasemi, Sm Abtahi Froushani, A Ownagh ,
Volume 21, Issue 11 (2-2017)
Abstract
Background and aim: Zymosan-induced peritonitis model can use to study the recruitment of monocytes and neutrophils into the peritoneal cavity and to study the effects of existing and novel anti-inflammatory drugs. This study was conducted to evaluate the effects of hydroalcoholic extract of Hypericum Perforatum on acute peritonitis induced by Zymosan in NMRI mice.
Methods: Fifty male NMRI mice were randomly allocated in 10 equal gropes and treated with 0 ,100, 200 or 400 mg/kg of hydroalcoholic extract of H. Perforatum and or 10mg/kg diclofenac 1 hours before the induction of peritonitis. To induce peritonitis, each mouse intraperitoneally received 10 µg of zymosan in 0.4 ml of saline. After 48 h, the peritoneal cavity was lavaged by 5 ml of cold PBS and the isolated cells were used to evaluate cell differential count, nitric oxide production and severity of respiratory burst. Statistical dada tests were analyzed using the Kruskal-Wallis test, Mann-Whitney and Bonferroni adjustment.
Results: The data showed that the nitric oxide and respiratory burst which was produced from exudate cells by peritoneal lavage in mice that received H. Perforatum at doses of 200 and 400 mg/kg or diclofenac compared to mice received normal saline were reduced. Total cell number in peritoneal cavity significantly decreased in all treatment group. However, no significant difference was observed between treatment groups with Hypericum perforatum extract. Using diclofenac or hydroalcoholic extract of H. Perforatum caused a significant decrease in the levels of TNF-α, IL-1 and IL-6. Diclofenac caused more profound reduction in the levels of TNF-α, IL-1 compared to extract. Nevertheless, the level of IL-6 was indicated a significant decrease in mice with peritonitis received hydroalcoholic extract especially in dose 400 mg/kg compared to mice with peritonitis received diclofenac.
Conclusion: In total it seems that the hydroalcholic extract of H. Perforatum may be a suitable as natural source to control inflammation caused by acute peritonitis.
F Farshbaf Manei Sefat, A Abolghasemi , U Barahmand , N Hajloo,
Volume 22, Issue 1 (4-2017)
Abstract
ABSTRACT
Background & aim: Menstruation as an important issue in adolescence and menstrual pain is a common problem in adolescents. Regarding the relationship between pain severity and pain self-efficacy, this study aimed to investigate and compare the efficacy of cognitive-behavioral therapy and hypnosis therapy on pain and pain self-efficacy in girls with primary dysmenorrhea.
Methods: The method of research is Quasi experimental and research design is pretest- posttest with control group. The study population included all second high school girls with primary dysmenorrhea in Ardabil city on 2015. a sample of 1,600 girls was screened by multi-stage cluster sampling method. After checking of entry criteria, 75 of those were assigned in 3 groups (two experimental groups and one control group) randomly. Self-efficacy questionnaire, visual analogue and scale Researcher- made questionnaire was used for collection data in pre-test and post-test stage. In order to collect data on pre and post grading, visual analog scale pain self-efficacy and self-administered questionnaire was used. For experimental groups, Pain control training based on cognitive-behavioral approach and self-hypnotism training was executed at 10 sessions in 45 minutes whereas control groups was in waiting list. Data collected were analyzed by descriptive statistics (mean and standard deviation) and multivariate analysis of covariance, Kruskal-Wallis and Mann-Whitney U.
Results: According to the chi Eta in one way covariance analysis The effectiveness of therapies on pain self-efficacy was 49 percent. There was no significant difference between cognitive behavioral group and hypnotherapy on pain self-efficacy justified mean with Bonferroni correction but there was significant difference between cognitive behavioral group and control group and between hypnotherapy group and control group on pain self-efficacy justified mean. The results of Kruskal Wallis showed that there is significant difference between 3 groups in pain severity. For comparison amongs groups, U-Mann Whitney index showed that the pain severity reduced significantly in cognitive behavioral and hypnotherapy group compared with control group and hypnotherapy which is More effective than cognitive behavioral therapy.
Conclusion: Cognitive behavioral therapy and hypnotherapy are effective in increased pain self-efficacy and reducing pain severity in girls with primary dysmenorrhea.
R Ghasemi , F Mir Mohammad Rezaei , S Nasri , A Nili Ahmadabadi ,
Volume 22, Issue 4 (10-2017)
Abstract
Abstract
Background and aim: Organophosphate insecticide diazinon is used in agriculture and may affect fertility of men due to the production of free radicals. Because of the antioxidant properties of black currant compounds, the effect of hydroalcoholic extract of black currant on spermatogenesis in diazinon-induced toxicity in male rats was investigated.
Methods: In the present experimental study, 30 adult male Wistar rats were divided into six groups: control group without sham, corn oil and saline without intervention; the positive control group received Diazinon poison at a concentration of 16 mg / kg. Experimental 1 and 2 respectively received diazinon poison (16 mg / kg) and black and white extract (100 mg / kg) and 200 mg / kg (3 mg / kg) extract, respectively. They received 3 extracts of black currant with a concentration of 200 mg / kg. All treatments were carried out for 2 months in gavage. After 2 months sperm parameters, number, mobility, life and morphology were investigated. Similarly, testicular tissue was stained with Hematoxylin Eosin for evaluation of spermatogenesis after cutting. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Results: Diazinon with a concentration of 16 mg / kg caused a significant decrease in the number and motility of the sperm in comparison to the sham group (p <0.01) (p <0.001), but significantly altered sperm motility and morphology compared to the group Did not have you The group that received diazinon and extract of black currant with two concentrations of 100 and 200 mg / kg compared to diazinon group showed a significant increase in the number of sperms (p <0.01) (p <0.001), but blackheads caused a significant change Sperm motility was not present in the presence of diazinone. Histological examination showed that the number of primary spermatocyte cells and spermatid in the diazinone group compared to sham group decreased significantly (p <0.05). However, the number of spermatogonia cells, primary spermatocytes and spermatid in experimental groups 1 and 2 showed a significant increase compared to diazinon group (p <0.05). In the experimental group 3, the extract increased the number of sperm in comparison with the sham group (p <0.05), but did not change the testicular tissue.
Conclusion: The results indicated that black currant extract had a protective effect on testicular tissue and could increase spermatogonia, spermatocyte, spermatid and sperm count in diazinon-induced toxicity but did not affect sperm motility.
T Ghasemipour , A Salehzadeh, Sa S Adat Shandiz ,
Volume 22, Issue 4 (10-2017)
Abstract
Abstract
Background and aim: The emergence of nanotechnology is one of the most promising areas for medical research. Today, biological methods of synthesizing nanoparticles have been considered in the fight against many diseases. The purpose of this study was to evaluate the anti-cancer and anti-bacterial activity of silver nanoparticles, biosynthesized with cyanobacteria acetate extract.
Methods: In the present experimental study, the silver nanoparticles biosynthesis was performed using silver ions regeneration with cyanobacteria acetate extracts. Techniques such as X-ray diffraction, scanning electron microscopy and transient evaluation of silver nanoparticles were evaluated. In order to investigate the antibacterial activity of synthesized nanosilver, serial dilution method was used for broth microdilution test to determine minimum inhibitory concentration (MIC). The effects of silver nanoparticle toxicity on T47D breast cancer cell line were evaluated using MTT colorimetric method. Also, the proximal anxine 0.5 propidoid yodide kit and flow cytometry system were evaluated to evaluate the percentage of apoptosis and necrosis in cancer cells treated with silver nanoparticles.
Results: Characterization of biosynthetic silver nanoparticles indicated that these nanoparticles had a mean size of 30 nm with dominant spherical morphology. The evaluation of the antibacterial properties of biosynthetic nanoparticles showed that the minimum inhibitory concentration for Escherichia coli, Acinetobacter Bumanni and Staphylococcus aureus was 25, 50 and 12.5 μg / ml, respectively. The results of cell proliferation of nanoparticles showed that its effect depends on the concentration and time of treatment of silver nanoparticles on cancerous cells. In addition, flow cytometric results showed an apoptotic cell death rate of 35% in the T47D cell line.
Conclusion: Biosynthesis nanoparticles have anticancer and antibacterial activity and can be studied further in the treatment of breast cancer and infections caused by pathogenic bacteria.
H Mehrani Bainvig , N Gholamzadeh , R Ghalandari , F Ghasemi,
Volume 23, Issue 5 (11-2018)
Abstract
Background & aim: The use of cyclophosphamide in the treatment of some diseases with its adverse side effects is imperative. Considering the well-known value of cinnamon extract, the present study aimed to evaluate its protective effect against the effects of cyclophosphamide on ovarian function in mice
Methods: forty-nine adult rats were arranged in seven groups. No treatment control group, sham group was injected drug’s solvent, and treatment groups 1, 2, 3 were injected 5mg/kg/b.wt Cyclophosphamide daily. These groups were gavaged )0.5, 1, 2(mg/kg/b.wt Cinnamon extract daily. Group 4 was injected 5 mg/kg/b.wt Cyclophosphamide, and Group 5 was gavaged 2 mg/kg/b.wt Cinnamon extract. After 21 days, all rats were unconscious and blood was taken from their hearts. Immediately, the left ovary of them was removed and after fixation in 10% formalin solution and tissue processing, the sections with 5 micron thickness were prepared and studied. By centrifuging the blood, serum was isolated and the concentration of FSH and LH, estrogen and progesterone hormones was measured by ELISA method. The obtained data were analyzed with SPSS (18( and ANOVA, and they were compared with Duncan’s test.
Results: The study of prepared photomicrographs of ovarian tissue indicated that the mean of follicle types and corpus luteum presented a significant decline in the treatment of Cyclophosphamide and Cinnamon extract especially in maximum dose compared to the control group and increase compared to the treatment Cyclophosphamide (P<0.05). Compared to cyclophosphamide treatment, there was a significant increase in the level of P <0.05. Extract of cinnamon extract increased significantly compared to other groups. Significant increase in atherosclerosis was observed in cyclophosphamide treatment and its reduction in other treatments. Significant decrease in FSH and LH, estrogen and progesterone in cyclophosphamide treatment and their increase in other treatments were observed.
Conclusion: According to these results, the cinnamon extract with its antioxidant properties had a dose- dependent protective effect on the pituitary-gond axis, and by increasing the number of follicles and the level of ovarian hormones, it reduced the adverse effects of cyclophosphamide in the ovary. Therefore, further research is recommended on generalizing this result to humans.
S Azizi , A Movahedi , E Arabameri , A Ghasemi ,
Volume 24, Issue 1 (4-2019)
Abstract
Background & aim: Parkinson's disease is a disorder in the nervous system due to the degeneration of dopaminergic neurons. Dopaminergic system is susceptible to neurological disorders caused by exposure to stressors early. Positive experiences are one of the most powerful treatments to increase neuroplasticity and motor function at any time of life. The purpose of the present study was to investigate the effect of pre-treatment endurance training on the balance of Parkinson's rats with perinatal stress.
Methods: In the present experimental study, 48 neonates rats of 30 days of age, with half of them under perinatal stress, were randomly divided into 8 groups consistening of no stress groups (control, sham, parkinson, endurance training + Parkinson's) and stress groups (control, Sham, Parkinson's, Endurance Exercise + Parkinson's). After 8 weeks of endurance training, stereotoxic surgery was performed on mice to create a Parkinson's model. After three weeks, theapomorphine test was used for diagnosis of Parkinson's disease and balance beam equilibrium test. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Results: The results indicated that the endurance exercises improved balance in both groups with and without prenatal stress(p<0.05). Furthermore, prenatal stress reduced balance, but this reduction was not significant(p=0.059), and perinatal stress also reduced the beneficial effects of exercise on balance test(p <0.05).
Conclusion: According to the findings, perinatal stress seems to reduce the balance of Parkinson's rats and endurance exercises reduce the negative effects of perinatal stress, which leads to a reduction in the negative effects of Parkinson's on balance.
|
|
