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Background & aim: In vitro maturation and fertilization of oocytes play an important role in reproductive biotechnology. The aim of this study is to define the IGF-1 effect on in vitro maturation, fertilization and development of mice immature oocytes to 2-cells in TCM199 medium cultures. Methods: In this study 4 week old NMRI mice were used. Ovaries stimulation carried out using PMSG. GV oocytes with or without cumulus cells were isolated from ovaries and cultured in TCM199 in presence of 100 ng IGF-1 for 24hr.The oocytes (MII) were inseminated with sperm in T6 medium for fertilization and development of 2-cells stage and they were investigated under inverted microscope. Data analysis was performed by using Chi- 2 test. Results: In cumulus cell group and in the presence of insulin-like growth factor fertilization of oocytes, forming embryos and the formation of 2-cells compared to the group without cumulus cells significantly increased (p < 0.05). Conclusion: As the results showed oocytes with cumulus cells in the presence of insulin-like growth factor enhances maturation, fertilization and embryonic development in 2-cells oocytes compared to group without cumulus cells TCM199.

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Background & aim: The effectiveness of the Transtheoretical model for changing nutritional behavior has been questioned by some studies. The purpose of this study was to investigate the relationship between self-efficacy and decisional equilibrium and transfer among the stages of behavior change of a calorie balance intake among obese women inYasuj, Iran. Methods : The data of the present descriptive analytical study were baseline data of 447 obese or overweight who were selected using the random cluster method. Based on the Transtheoretical model, individuals pass stages of change, which are precontemplation, contemplation, preparedness, action and maintenance. Two other main constructs which affect the stages of behavior change are self-efficacy and decisional balance. Demographic and anthropometric variables and stages of change, self-efficacy, decisional equilibrium pros and cons related to calorie balance intake were measured using valid and reliable questionnaires. Results: The participants average age was 33.49 years. Neither demographic nor anthropometric variables changes were significant among the stages of behavior change except literacy level before high school ( P=.047,OR=.502, %95 CI=.255 ~ .990). A significant change was observed in self-efficacy ( F(4.425)= 27.09, P= .000),decisional equilibrium (F(4.394), P= .004), and pros (F(4.430)=5.33, P=000), while no significant change was observed among cons (F(4.400)=1.83, P=.123). Conclusion: The findings confirmed the specific model of self-efficacy, decisional equilibrium and pros which predict that the obese women transfer between the five stages of calorie balance intake.

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Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes.

Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test.

Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P <0.001).

Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, &beta-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with cumulus cells more than the oocyte without cumulus cells to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with cumulus cells.

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Background & Aim: Nutritional literacy is the capacity of people to gain, understand and evaluate nutritional information for proper nutrition decision making. This research has been designed and implemented to measure the nutritional literacy of primary school teachers in Yasuj

 

Methods: This cross-sectional study was performed on two parts of the nationalization of the Nutrition Literacy Instrument developed in Turkey, as well as nutritional literacy assessment in elementary school teachers in Yasuj. The selected instrument was localized using translate translation method and receiving and applying opinions of nutritionists specialist on the structure and content of the questionnaire, and then ensuring its reliability for use in Iranian society. Nutritional literacy data were collected from a group of 110 elementary teachers in Yasouj who were selected by multi-stage cluster sampling through a self-administered questionnaire and then analyzed by descriptive and analytical statistics.

 

Results: The localized nutritional literacy tool had 35 items and the Cronbach's alpha coefficient was 0.73. Mean and standard deviation of teacher's score from the nutritional literacy tool was 27.14 ± 3.2, which indicated that 22.7% of teachers had inadequate nutritional literacy. Teachers with fewer years of work and higher education had a higher level of nutritional literacy. The lowest percentages of responses were related to the skill of determining food units, doing calculations and reading food labels.

 

Conclusions: Majority of the teachers studied had sufficient nutritional literacy, but defect in three parts of the skill of determining food units, doing calculations and reading food labels indicates the need to pay attention to these components in the design and implementation of nutrition improvement programs.