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Anti-leishmanial activity of Carthamus tinctorius and Matricaria chamomilla extracts on the survival of promastigotes of Leishmania major
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Sahar Hamoonnavard1   , Hossein Rezvan2 , Maral Maleki2 , Hamidreza Heydarianpour2 , Behnaz Bazargani-Gilani2 , Fatemeh Behdarvand2 |
1- Bu-Ali Sina University , s.hamoonnavard@basu.ac.ir
2- Bu-Ali Sina University |
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Abstract: (414 Views) |
Introduction: The side effects and resistance to chemical treatment of cutaneous leishmaniasis have led to intensive research into the use of herbal compounds for the treatment of this disease. The antimicrobial properties of Carthamus tinctorius and Matricaria chamomilla extract have been investigated on many microorganisms. The aim of this study was to investigate the anti-leishmanial effect of these two plants on the promastigotes of the Leishmania major.
Materials and methods: The standard strain of Leishmania major was obtained from Pasteur Institute of Iran. Ethanol extract of safflower and water-ethanol extract of chamomile were prepared at concentrations of 15, 25, 50, 75, 100 and 200 μg/ml and exposed to promastigotes cultured in 96 plates in triplicate and incubated for 24 hours. Using colorimetric method and tetrazolium reduction, the viability of promastigotes was evaluated by absorbance at 490nm. The inhibitory concentrations(IC) IC10, IC50, IC80 each of the extracts were calculated with Graphpad Prism 8 statistical software.
Findings: Exposure to safflower and chamomile extracts at all concentrations decreased parasite survival(p≤0.05), such that inhibitory concentrations was IC10: 21.10ug/ml, IC50: 3.85ug/ml and IC80 1.31ug/ml for safflower and inhibitory concentrations was IC10: 24.27ug/ml, IC50: 17.69ug/ml, IC80: 14.48ug/ml for chamomile extract. Concentrations of more than 15 μg/ml and more than 25μg/ml had significant anti-leishmanial effects compared to the untreated control (p≤0.05).
Conclusion: The anti-leishmanial effect of safflower extract and chamomile extract on Leishmania major was remarkable at low concentrations and can be investigated as a herbal compound in the in-vivo model of cutaneous leishmaniasis.
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| Keywords: Cutaneous leishmaniasis, Leishmania major, Carthamus tinctorius, Matricaria chamomilla, Viability |
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Type of Study: Research |
Subject:
Parasitology
Received: 2024/12/7 | Accepted: 2025/02/19 | Published: 2025/03/5
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